超清版 GB/T 44350-2024 油菜蜂蜜中丁香酸甲酯的测定 高效液相色谱法
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- 更新时间：2025-03-18
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资料介绍
ICS 67.180.10
CCS B 47
中华人民共和国国家标准
GB/T 44350—2024
油菜蜂蜜中丁香酸甲酯的测定
高效液相色谱法
Determination of methyl syringate in rape honey—High performance liquid
chromatography method
2024-09-29 发布2025-04-01 实施
国家市场监督管理总局
国家标准化管理委员会发 布
目 次
前言 ····································································································· Ⅲ
1 范围 ·································································································· 1
2 规范性引用文件 ······················································································ 1
3 术语和定义 ··························································································· 1
4 原理 ·································································································· 1
5 试剂和材料 ··························································································· 1
6 仪器设备 ······························································································ 2
7 分析步骤 ······························································································ 2
7.1 试样制备 ························································································· 2
7.2 空白试验 ························································································· 2
7.3 平行试验 ························································································· 2
7.4 测定 ······························································································ 2
8 结果计算 ······························································································ 3
9 定量限 ································································································ 3
10 精密度 ······························································································· 3
附录A （资料性） 丁香酸甲酯标准工作液的高效液相色谱图 ········································ 4
前 言
本文件按照GB/T 1.1—2020《标准化工作导则 第1 部分：标准化文件的结构和起草规则》的规
定起草。
请注意本文件的某些内容可能涉及专利。本文件的发布机构不承担识别专利的责任。
本文件由中华全国供销合作总社提出。
本文件由全国蜂产品标准化技术委员会（SAC/TC 601）归口。
本文件起草单位：中国计量大学、安徽蜜之源食品集团有限公司、广州金和技术检测有限公司、中
科标准（宁德）科技有限公司、深圳市威标检测技术有限公司、南京海关动植物与食品检测中心、金华
市农业科学研究院、中国农业科学院蜜蜂研究所、浙江省乡村振兴促进中心、绿城农科检测技术有限公
司、浙江华才检测技术有限公司、浙江省畜牧技术推广与种畜禽监测总站、江苏国测检测技术有限公
司、深圳昱朋科技有限公司、齐齐哈尔市检验检测中心、青岛中一监测有限公司、湖州市蜂业研究院。
本文件主要起草人：李红亮、朱鑫麗、林宏、苏晓玲、吴帆、乔江涛、黄金晶、王志军、林钗、
何丽、李加芬、汪腊云、李奎、林影、黄泳铭、王韦岗、娄喜山、钟巧霞、邱凯。
1 范围
本文件描述了测定油菜蜂蜜中丁香酸甲酯含量的高效液相色谱方法。
本文件适用于油菜蜂蜜中丁香酸甲酯含量的测定。
2 规范性引用文件
下列文件中的内容通过文中的规范性引用而构成本文件必不可少的条款。其中，注日期的引用文
件，仅该日期对应的版本适用于本文件；不注日期的引用文件，其最新版本（包括所有的修改单）适用
于本文件。
GB/T 6682 分析实验室用水规格和试验方法
3 术语和定义
本文件没有需要界定的术语和定义。
4 原理
油菜蜂蜜样品中的丁香酸甲酯经弱阴离子交换固相萃取柱净化和富集后，经C18 高效液相色谱柱分
离，紫外或二极管阵列检测器检测（最大吸收波长为276 nm），外标峰面积法定量。
5 试剂和材料
除非另有说明，所有试剂均为分析纯，水为 GB/T 6682 规定的一级水。
5.1 乙腈（色谱纯）。
5.2 氨水：浓度为 25%～28%（质量
CCS B 47
中华人民共和国国家标准
GB/T 44350—2024
油菜蜂蜜中丁香酸甲酯的测定
高效液相色谱法
Determination of methyl syringate in rape honey—High performance liquid
chromatography method
2024-09-29 发布2025-04-01 实施
国家市场监督管理总局
国家标准化管理委员会发 布
目 次
前言 ····································································································· Ⅲ
1 范围 ·································································································· 1
2 规范性引用文件 ······················································································ 1
3 术语和定义 ··························································································· 1
4 原理 ·································································································· 1
5 试剂和材料 ··························································································· 1
6 仪器设备 ······························································································ 2
7 分析步骤 ······························································································ 2
7.1 试样制备 ························································································· 2
7.2 空白试验 ························································································· 2
7.3 平行试验 ························································································· 2
7.4 测定 ······························································································ 2
8 结果计算 ······························································································ 3
9 定量限 ································································································ 3
10 精密度 ······························································································· 3
附录A （资料性） 丁香酸甲酯标准工作液的高效液相色谱图 ········································ 4
前 言
本文件按照GB/T 1.1—2020《标准化工作导则 第1 部分：标准化文件的结构和起草规则》的规
定起草。
请注意本文件的某些内容可能涉及专利。本文件的发布机构不承担识别专利的责任。
本文件由中华全国供销合作总社提出。
本文件由全国蜂产品标准化技术委员会（SAC/TC 601）归口。
本文件起草单位：中国计量大学、安徽蜜之源食品集团有限公司、广州金和技术检测有限公司、中
科标准（宁德）科技有限公司、深圳市威标检测技术有限公司、南京海关动植物与食品检测中心、金华
市农业科学研究院、中国农业科学院蜜蜂研究所、浙江省乡村振兴促进中心、绿城农科检测技术有限公
司、浙江华才检测技术有限公司、浙江省畜牧技术推广与种畜禽监测总站、江苏国测检测技术有限公
司、深圳昱朋科技有限公司、齐齐哈尔市检验检测中心、青岛中一监测有限公司、湖州市蜂业研究院。
本文件主要起草人：李红亮、朱鑫麗、林宏、苏晓玲、吴帆、乔江涛、黄金晶、王志军、林钗、
何丽、李加芬、汪腊云、李奎、林影、黄泳铭、王韦岗、娄喜山、钟巧霞、邱凯。
1 范围
本文件描述了测定油菜蜂蜜中丁香酸甲酯含量的高效液相色谱方法。
本文件适用于油菜蜂蜜中丁香酸甲酯含量的测定。
2 规范性引用文件
下列文件中的内容通过文中的规范性引用而构成本文件必不可少的条款。其中，注日期的引用文
件，仅该日期对应的版本适用于本文件；不注日期的引用文件，其最新版本（包括所有的修改单）适用
于本文件。
GB/T 6682 分析实验室用水规格和试验方法
3 术语和定义
本文件没有需要界定的术语和定义。
4 原理
油菜蜂蜜样品中的丁香酸甲酯经弱阴离子交换固相萃取柱净化和富集后，经C18 高效液相色谱柱分
离，紫外或二极管阵列检测器检测（最大吸收波长为276 nm），外标峰面积法定量。
5 试剂和材料
除非另有说明，所有试剂均为分析纯，水为 GB/T 6682 规定的一级水。
5.1 乙腈（色谱纯）。
5.2 氨水：浓度为 25%～28%（质量