超清版 GB/T 44396-2024 化学品 稀有鮈鲫胚胎急性毒性试验
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- 更新时间：2025-03-18
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资料介绍
ICS 13.300
CCS A 80
GB/T 44396—2024
化学品 稀有鮈鲫胚胎急性毒性试验
Chemicals—Rare minnow（ Gobiocypris rarus） embryo acute toxicity test
2024⁃08⁃23 发布2025⁃03⁃01 实施
国家市场监督管理总局
国家标准化管理委员会发 布
目 次
前言··························································································································Ⅲ
1 范围·······················································································································1
2 规范性引用文件········································································································1
3 术语和定义··············································································································1
4 受试物信息··············································································································1
5 试验原理·················································································································2
6 设备与材料··············································································································2
7 试验前期准备···········································································································3
8 试验程序·················································································································4
9 质量控制·················································································································6
10 数据与报告············································································································7
附录A（ 资料性） 稀有鮈鲫胚胎的正常发育图····································································8
附录B（ 资料性） 试验用水水质条件·················································································9
附录C（ 资料性） 稀有鮈鲫胚胎急性毒性试验鱼卵鉴别参考图··············································10
附录D（ 资料性） 24 孔细胞培养板布局···········································································12
参考文献····················································································································13
前 言
本文件按照GB/T 1.1—2020《标准化工作导则 第1 部分：标准化文件的结构和起草规则》的规
定起草。
请注意本文件的某些内容可能涉及专利。本文件的发布机构不承担识别专利的责任。
本文件由全国危险化学品管理标准化技术委员会（SAC/TC 251）提出并归口。
本文件起草单位：上海市检测中心、生态环境部固体废物与化学品管理技术中心、中国科学院水生
生物研究所、沈化测试技术（南通）有限公司、宁波海关技术中心、苏州华测安评技术服务有限公司、
贵州健安德科技有限公司、广东省科学院微生物研究所（广东省微生物分析检测中心）。
本文件主要起草人：陈晓倩、杨婧、张瑛、刘亚楠、殷浩文、刘洪英、刘晓建、王剑伟、何勇凤、李子璇、
刘汉伟、章跃龙、皮天星、田宝莲、杨鸿波、董榕贵、吴欣颖。
1 范围
本文件描述了化学品对稀有鮈鲫胚胎急性毒性试验方法，包括受试物所需信息、试验原理、仪器设
备、试验前期准备、试验程序、质量控制、数据与报告。
本文件适用于测试化学品对稀有鮈鲫胚胎的急性毒性效应。
2 规范性引用文件
下列文件中的内容通过文中的规范性引用而构成本文件必不可少的条款。其中，注日期的引用文
件，仅该日期对应的版本适用于本文件；不注日期的引用文件，其最新版本（包括所有的修改单）适用于
本文件。
GB/T 27861 化学品 鱼类急性毒性试验
3 术语和定义
GB/T 27861 界定的以及下列术语和定义适用于本文件。
3.1
静态试验 static test
以整个试验过程中试验介质不更换、不流动的方式进行的试验。
3.2
半静态试验 semi⁃static test
以定期（如24 h）更换试验介质的方式进行的试验。
3
CCS A 80
GB/T 44396—2024
化学品 稀有鮈鲫胚胎急性毒性试验
Chemicals—Rare minnow（ Gobiocypris rarus） embryo acute toxicity test
2024⁃08⁃23 发布2025⁃03⁃01 实施
国家市场监督管理总局
国家标准化管理委员会发 布
目 次
前言··························································································································Ⅲ
1 范围·······················································································································1
2 规范性引用文件········································································································1
3 术语和定义··············································································································1
4 受试物信息··············································································································1
5 试验原理·················································································································2
6 设备与材料··············································································································2
7 试验前期准备···········································································································3
8 试验程序·················································································································4
9 质量控制·················································································································6
10 数据与报告············································································································7
附录A（ 资料性） 稀有鮈鲫胚胎的正常发育图····································································8
附录B（ 资料性） 试验用水水质条件·················································································9
附录C（ 资料性） 稀有鮈鲫胚胎急性毒性试验鱼卵鉴别参考图··············································10
附录D（ 资料性） 24 孔细胞培养板布局···········································································12
参考文献····················································································································13
前 言
本文件按照GB/T 1.1—2020《标准化工作导则 第1 部分：标准化文件的结构和起草规则》的规
定起草。
请注意本文件的某些内容可能涉及专利。本文件的发布机构不承担识别专利的责任。
本文件由全国危险化学品管理标准化技术委员会（SAC/TC 251）提出并归口。
本文件起草单位：上海市检测中心、生态环境部固体废物与化学品管理技术中心、中国科学院水生
生物研究所、沈化测试技术（南通）有限公司、宁波海关技术中心、苏州华测安评技术服务有限公司、
贵州健安德科技有限公司、广东省科学院微生物研究所（广东省微生物分析检测中心）。
本文件主要起草人：陈晓倩、杨婧、张瑛、刘亚楠、殷浩文、刘洪英、刘晓建、王剑伟、何勇凤、李子璇、
刘汉伟、章跃龙、皮天星、田宝莲、杨鸿波、董榕贵、吴欣颖。
1 范围
本文件描述了化学品对稀有鮈鲫胚胎急性毒性试验方法，包括受试物所需信息、试验原理、仪器设
备、试验前期准备、试验程序、质量控制、数据与报告。
本文件适用于测试化学品对稀有鮈鲫胚胎的急性毒性效应。
2 规范性引用文件
下列文件中的内容通过文中的规范性引用而构成本文件必不可少的条款。其中，注日期的引用文
件，仅该日期对应的版本适用于本文件；不注日期的引用文件，其最新版本（包括所有的修改单）适用于
本文件。
GB/T 27861 化学品 鱼类急性毒性试验
3 术语和定义
GB/T 27861 界定的以及下列术语和定义适用于本文件。
3.1
静态试验 static test
以整个试验过程中试验介质不更换、不流动的方式进行的试验。
3.2
半静态试验 semi⁃static test
以定期（如24 h）更换试验介质的方式进行的试验。
3